Frederic PINCET

Membrane biophysics
Department of Physics
, updated on
14 November 2021
1er étage, bureau GH107
24, rue Lhomond 75231 Paris cedex 05
01 44 322502

I am trained as a physicist and I study membrane molecular mechanisms involved in biological/physiological processes.

Field of research

The team conducts research at the Physical/Biological interface, aiming to describe fundamental biological phenomena in a quantitative and predictive manner. Its main themes are interactions, dynamics and membrane rearrangements at the origin of cell adhesion and membrane fusion in neurotransmission and fertilization. We characterize the intermediate states of these processes in terms of energy, structure, molecular organization and membrane dynamics, by combining advanced physical approaches and tools (quantification of molecular bonds and membrane adhesion by micromanipulation of individual objects, quantitative imaging, microfluidics, biochemistry, cell biology and by developing biomimetic models inspired by the physics of soft matter.


20 publications des 10 dernières années

  1. P. Heo, J. Coleman, J.-B. Fleury, J.E. Rothman, F. Pincet, “Nascent fusion pore opening monitored at single-SNAREpin resolution”, PNAS, 118: e2024922118 (2021).
  2. C. Gruget, O. Bello, J. Coleman, S.S. Krishnakumar, E. Perez, J.E. Rothman, F. Pincet & S.H. Donaldson Jr, “Synaptotagmin‑1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain”, Scientific Reports, 10:18011 (2020)
  3. P. Heo, and F. Pincet, “Freezing and piercing of in vitro asymmetric plasma membrane by α-synuclein”, Communications Biology, 3: 148 (2020).
  4. P. Heo, S. Ramakrishnan, J. Coleman, J.E. Rothman, J.-B. Fleury, and F. Pincet, “Highly Reproducible Physiological Asymmetric Membrane with Freely Diffusing Embedded Proteins in a 3D-Printed Microfluidic Setup”, Small, 2019: 1900725 (2019).
  5. S. Ramakrishnan, A. Gohlke, F. Li, J. Coleman, W. Xu, J.E. Rothman, F. Pincet, “High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated Analysis”, Langmuir, 34 : 5849-5859 (2018).
  6. M.W. Grome, Z. Zhang, F. Pincet, C.X. Lin, “Vesicle Tubulation with Self-Assembling DNA Nanosprings”, Angewandte Chemie, 57,5330-5334 (2018).
  7. Z. Zhang, Y. Yang, F. Pincet, M. C. Llaguno, C. Lin, “Placing and shaping liposomes with reconfigurable DNA nanocages”, Nature Chemistry, 9 : 653-659 (2017).
  8. C. François-Martin, F. Pincet, “Actual fusion efficiency in the lipid mixing assay - Comparison between nanodiscs and liposomes”, Scientific Reports, 7: 43860 (2017).
  9. C. François-Martin, J.E. Rothman, F. Pincet, “Low energy cost for optimal speed and control of membrane fusion”, PNAS, 114: 1238–1241 (2017).
  10. J. Di Russo, A.-L. Luik, L. Yousif, S. Budny, H. Oberleithner, V. Hofschroeer, J. Klingauf, E. van Bavel, E.N. Bakker, P. Hellstrand, A. Bhattachariya, S. Albinsson, F. Pincet, R. Hallmann, L.M. Sorokin “Endothelial basement membrane laminin 511 is essential for shear stress response”, EMBO Journal, 36: 183–201 (2017).
  11. F. Li, N. Tiwari, J.E. Rothman, F. Pincet, “Kinetic barriers to SNAREpin assembly in the regulation of membrane docking/priming and fusion”, PNAS, 113: 10536–10541 (2016).
  12. Y. Saheki, X. Bian, C.M. Schauder, Y. Sawaki, M.A. Surma, C. Klose, F. Pincet, K.M. Reinisch, P. De Camilli, “Control of plasma membrane lipid homeostasis by the extended synaptotagmins”, Nature Cell Biology, 18: 504-515 (2016).
  13. Y.J. Wang, F. Li, N. Rodriguez, X. Lafosse, C. Gourier, E. Perez, F. Pincet, “Snapshot of sequential SNARE assembling states between membranes shows that N-terminal transient assembly initializes fusion”, PNAS, 113: 3533-3538 (2016).
  14. W. Xu, J. Wang, J.E. Rothman, F. Pincet, “Accelerating SNARE-mediated Membrane Fusion by DNA-lipid Tethers”, Angewandte Chemie, 54,14388-14392 (2015).
  15. I. Motta, A. Gohlke, V. Adrien, F. Li, H. Gardavot, JE Rothman, F. Pincet, "Formation of Giant Unilamellar Proteo-Liposomes by Osmotic Shock"  Langmuir, 31 : 7091-7099 (2015).
  16. F. Li, D. Kuemmel, J. Coleman, K. M. Reinisch, J. E. Rothman, F. Pincet, "A Half-Zippered SNARE Complex Represents a Functional Intermediate in Membrane Fusion", J. Am. Chem. Soc., 136, 3456-3464 (2014).
  17. J. Delacotte, C. Gourier, F. Pincet, "Interfacial pressure and phospholipid density at emulsion droplet interface using fluorescence microscopy", Coll. Surf. Sc. B., 117: 545-548 (2014).
  18. A.-R. Thiam, B. Antonny, J. Wang, J. Delacotte, F. Wilfling, T. C. Walther, R. Beck, J.E. Rothman, F. Pincet, "COPI buds 60 nm lipid droplets from reconstituted water-phospholipid-triacylglyceride interfaces, suggesting a tension clamp function. ", PNAS, 110:13244-13249 (2013).
  19. L. Shi, K. Howan, Q.-T. Shen, Y. J. Wang, J.E. Rothman, F. Pincet, "Preparation and characterization of SNARE-containing nanodiscs and direct study of cargo release through fusion pores", Nat. Prot., 8:935-948 (2013)
  20. L. Shi, Q.-T. Shen, A. Kiel, J. Wang, H.-W. Wang, T. J. Melia, J.E. Rothman, F. Pincet, "SNARE Proteins: One to Fuse and Three to Keep the Nascent Fusion Pore Open", Science; 335:1355-1359 (2012).