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Frederic PINCET

Chercheur
Biologie
Physique
Membrane biophysics
ENS-PSL
Département de physique
Publié le
15 juin 2021
, modifié le
14 novembre 2021
FPINCET
1er étage, bureau GH107
24, rue Lhomond 75231 Paris cedex 05
01 44 322502

Physicien de formation, j’étudie les mécanismes responsables de certains processus biologiques/physiologiques.

Champ de recherche

L’équipe mène une recherche à l’interface Physique/Biologie, visant à décrire de manière quantitative et prédictive des phénomènes biologiques fondamentaux. Ses principales thématiques portent sur les interactions, la dynamique et les remaniements membranaires à l’origine de l’adhésion cellulaire et la fusion membranaire dans la neurotransmission et la fécondation.

Elle caractérise les états intermédiaires de ces processus en termes d’énergie, de structure, d’organisation moléculaire et de dynamique de membrane, en combinant approches et outils physiques de pointe (quantification de lien moléculaires et d’adhésions membranaires par micromanipulation d’objets individuels, imagerie quantitative, microfluidique, biochimie, biologie cellulaire et en développant des modèles biomimétiques inspirés de la physique de la matière molle.

Publications

20 publications des 10 dernières années

  1. P. Heo, J. Coleman, J.-B. Fleury, J.E. Rothman, F. Pincet, “Nascent fusion pore opening monitored at single-SNAREpin resolution”, PNAS, 118: e2024922118 (2021).
  2. C. Gruget, O. Bello, J. Coleman, S.S. Krishnakumar, E. Perez, J.E. Rothman, F. Pincet & S.H. Donaldson Jr, “Synaptotagmin‑1 membrane binding is driven by the C2B domain and assisted cooperatively by the C2A domain”, Scientific Reports, 10:18011 (2020)
  3. P. Heo, and F. Pincet, “Freezing and piercing of in vitro asymmetric plasma membrane by α-synuclein”, Communications Biology, 3: 148 (2020).
  4. P. Heo, S. Ramakrishnan, J. Coleman, J.E. Rothman, J.-B. Fleury, and F. Pincet, “Highly Reproducible Physiological Asymmetric Membrane with Freely Diffusing Embedded Proteins in a 3D-Printed Microfluidic Setup”, Small, 2019: 1900725 (2019).
  5. S. Ramakrishnan, A. Gohlke, F. Li, J. Coleman, W. Xu, J.E. Rothman, F. Pincet, “High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated Analysis”, Langmuir, 34 : 5849-5859 (2018).
  6. M.W. Grome, Z. Zhang, F. Pincet, C.X. Lin, “Vesicle Tubulation with Self-Assembling DNA Nanosprings”, Angewandte Chemie, 57,5330-5334 (2018).
  7. Z. Zhang, Y. Yang, F. Pincet, M. C. Llaguno, C. Lin, “Placing and shaping liposomes with reconfigurable DNA nanocages”, Nature Chemistry, 9 : 653-659 (2017).
  8. C. François-Martin, F. Pincet, “Actual fusion efficiency in the lipid mixing assay - Comparison between nanodiscs and liposomes”, Scientific Reports, 7: 43860 (2017).
  9. C. François-Martin, J.E. Rothman, F. Pincet, “Low energy cost for optimal speed and control of membrane fusion”, PNAS, 114: 1238–1241 (2017).
  10. J. Di Russo, A.-L. Luik, L. Yousif, S. Budny, H. Oberleithner, V. Hofschroeer, J. Klingauf, E. van Bavel, E.N. Bakker, P. Hellstrand, A. Bhattachariya, S. Albinsson, F. Pincet, R. Hallmann, L.M. Sorokin “Endothelial basement membrane laminin 511 is essential for shear stress response”, EMBO Journal, 36: 183–201 (2017).
  11. F. Li, N. Tiwari, J.E. Rothman, F. Pincet, “Kinetic barriers to SNAREpin assembly in the regulation of membrane docking/priming and fusion”, PNAS, 113: 10536–10541 (2016).
  12. Y. Saheki, X. Bian, C.M. Schauder, Y. Sawaki, M.A. Surma, C. Klose, F. Pincet, K.M. Reinisch, P. De Camilli, “Control of plasma membrane lipid homeostasis by the extended synaptotagmins”, Nature Cell Biology, 18: 504-515 (2016).
  13. Y.J. Wang, F. Li, N. Rodriguez, X. Lafosse, C. Gourier, E. Perez, F. Pincet, “Snapshot of sequential SNARE assembling states between membranes shows that N-terminal transient assembly initializes fusion”, PNAS, 113: 3533-3538 (2016).
  14. W. Xu, J. Wang, J.E. Rothman, F. Pincet, “Accelerating SNARE-mediated Membrane Fusion by DNA-lipid Tethers”, Angewandte Chemie, 54,14388-14392 (2015).
  15. I. Motta, A. Gohlke, V. Adrien, F. Li, H. Gardavot, JE Rothman, F. Pincet, "Formation of Giant Unilamellar Proteo-Liposomes by Osmotic Shock"  Langmuir, 31 : 7091-7099 (2015).
  16. F. Li, D. Kuemmel, J. Coleman, K. M. Reinisch, J. E. Rothman, F. Pincet, "A Half-Zippered SNARE Complex Represents a Functional Intermediate in Membrane Fusion", J. Am. Chem. Soc., 136, 3456-3464 (2014).
  17. J. Delacotte, C. Gourier, F. Pincet, "Interfacial pressure and phospholipid density at emulsion droplet interface using fluorescence microscopy", Coll. Surf. Sc. B., 117: 545-548 (2014).
  18. A.-R. Thiam, B. Antonny, J. Wang, J. Delacotte, F. Wilfling, T. C. Walther, R. Beck, J.E. Rothman, F. Pincet, "COPI buds 60 nm lipid droplets from reconstituted water-phospholipid-triacylglyceride interfaces, suggesting a tension clamp function. ", PNAS, 110:13244-13249 (2013).
  19. L. Shi, K. Howan, Q.-T. Shen, Y. J. Wang, J.E. Rothman, F. Pincet, "Preparation and characterization of SNARE-containing nanodiscs and direct study of cargo release through fusion pores", Nat. Prot., 8:935-948 (2013)
  20. L. Shi, Q.-T. Shen, A. Kiel, J. Wang, H.-W. Wang, T. J. Melia, J.E. Rothman, F. Pincet, "SNARE Proteins: One to Fuse and Three to Keep the Nascent Fusion Pore Open", Science; 335:1355-1359 (2012).

Publications de Frederic PINCET