Integrative neuroscience, cortical dynamics, sensory coding, in vivo microscopy
Department of Biology
, updated on
16 March 2022
Institut de biologie de l’ENS (IBENS)
8ème étage
46, rue d'Ulm 75230 Paris cedex 05
01 44 323734

I study the cortical dynamics underlying sensory perception in behaving animals by developing innovative in vivo microscopy methods

Field of research

Our group studies neural networks dynamics underlying sensory coding, memory formation and representation of space. In particular, we study how attentional processes and expectancy mechanisms modulate the integration of tactile, auditory or visual information. We address these questions using advanced optical methods that we are continuously improving in terms of sensitivity, temporal resolution, depth penetration or adaptability to freely behaving animals. For example, we have developed two-photon microscopes allowing ultra-fast 2D and 3D recordings based on acousto-optic deflectors.


  1. Akemann W., Léger J.-F., Ventalon C., Mathieu B., Dieudonné S. and Bourdieu L., Fast spatial beam shaping by acousto-optic diffraction for 3D non-linear microscopy, (2015) Opt. Express 23(22), 28191-28205
  2. Estebanez L., Bertherat J., Shulz D.E., Bourdieu L., Léger J.-F., A radial map of multi-whisker correlation selectivity in the rat barrel cortex, (2016) Nature Comm. 7:13528
  3. Blochet B., Bourdieu L., Gigan S., Focusing light through dynamical samples using fast continuous wavefront optimization (2017) Opt. Lett., 42(23) 4994-4997
  4. Dussaux C., Fodor J., Szabo V., Chastagnier Y., Léger J.-F, Bourdieu L., Perroy J. and Ventalon C., Fast Confocal Fluorescence Imaging in Freely-Behaving Mice, Sci. Rep. (2018) 8(1):16262.
  5. Blochet B., Joaquina J, Blum L, Bourdieu L., Gigan S., Enhanced stability of the focus obtained by wavefront optimization in dynamical scattering media, Optica (2019) 6(12) 1554-1561
  6. Akemann W., Wolf S., Villette V., Ventalon C., Mathieu B., Léger J.-F., Dieudonné S. and Bourdieu L., Fast optical recording of neuronal activity by three-dimensional custom-access serial holography, Nat Methods (2022) 19, 100-110.