Protein stability, Biomembrane & responsive liposomes, Switchable polymer coatings
22 September 2021
, updated on
10 January 2022
1er étage, bureau E152
24, rue Lhomond 75231 Paris cedex 05
01 44 322403
Based on a core expertise in physical chemistry of polymers, surfactants, and soft matter science, I explore the design of stimuli-responsive (macro)molecular tools 1) in biochemistry and 2) in cell biology in tight collaboration with biologists.
Field of research
In collaboration with pharmaceutical companies, we study the stabilization of proteins in solution and in fluid polymer phases. Amphiphilic copolymers, including polyelectrolytes, are designed to bind to target proteins, and enhance their colloidal stability, and/or help refolding. We investigate the physical chemistry of protein aggregation and protein:polymer complexes.
We design stimuli-responsive polymers to explore dynamic switches (based on light, temperature or redox potential) on the surface biomembranes, including liposomes capsules and living cell membranes. Polymers studied with E. Marie, enable to prepare functional adlayers onto substrates and micropatterns to control soft adhesion, polarisation, migration of cells. With B. Miroux (IBPC), we develop the bacterial biosynthesis of polypeptide-coated responsive liposomes. One objective is to form and control artificial compartments in bacteria mutants expressing ad hoc (genetically encoded) sets of chimeric proteins.
- Marconnet, A.; Michon, B.; Le Bon, C.; Giusti, F.; Tribet, C.; Zoonens, M., Biomacromolecules 2020, 21 (8), 3459-3467, Solubilization and Stabilization of Membrane Proteins by Cycloalkane-Modified Amphiphilic Polymers..
- Royes J.; Ilioaia O.; Lubart Q.; Dubacheva G.; Bally M.; Miroux B.; Tribet C., Angew. Chem. Int. Ed., 2019, doi: 10.1002/anie.201902929, Protein-loaded Lipidic Nanocapsules from Bio-engineered Bacteria.
- Hespel, L.; Dupré de Baubigny, J.; Lalanne, P.; de Beco, S.; Coppey, M.; Villard, C.; Humblot, V.; Marie, E.; Tribet, C., ACS Applied Bio Materials 2019, 2 (10), 4367-4376Redox-Triggered Control of Cell Adhesion and Deadhesion on Poly(lysine)-g-poly(ethylene oxide) Adlayers.
- Martin N.; Costa N.; Wien F.; Winnik F.; Ortega C.; Herbet A.; Boquet D.; Tribet C., Macromolecular Biosciences, 2017, 17 (2) UNSP 1600213 DOI:10.1002/ mabi.201600213, Refolding of Aggregation-Prone ScFv Antibody Fragments Assisted by Hydrophobically Modified Poly(sodium acrylate) Derivatives.
- Stijn F. M. van Dongen, P. Maiuri, E. Marie, C. Tribet and M. Piel, Adv. Mater. 2013, 25(12):1687-91, Triggering Cell Adhesion, Migration or Shape Change with a Dynamic Surface Coating.